Linker- Based Sperm Mediated Gene Transfer Method for The
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منابع مشابه
O-10: Sperm Mediated Gene Transfer Using Adjuvant Preserving Fertility for Production of Transgenic Chicken Expressing
Background: Low uptake of exogenous DNA by sperm and reduced number of fertilized oocyte by transfected sperm are the major obstacles for progression of sperm mediated gene transfer. Therefore, the modification of sperm mediated gene transfer procedure needs to be required. The purpose of this study was to evaluate the efficiency of FuGene 6 compare to lipofection in transfection medium for int...
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Background: Many techniques have been developed to increase DNA molecules into sperms called sperm mediated gene transfer (SMGT) and one of them is electroporation. Sperm mediated gene transfer is an ideal method for mass gene transfer in organisms with external fertilization. Gene transfer is applied to rainbow trout sperm and the effects of electroporation on sperms and rate of gene uptake by...
متن کاملP-85: Epididymal Sperm Parameters following Sperm Mediated Gene Transfer of EDFP-pIRES Vector in Rat
Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...
متن کاملEffective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.
BACKGROUND Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the p...
متن کاملProviding a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles
Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare ...
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